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BioPro HIC, Hydrophobic Interaction Column, for protein - YMC

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BioPro HIC columns are capable of separating proteins under non-denaturing conditions. BioPro HIC HT offers a time-critical analysis of biopharmaceuticals such as antibody-drug conjugates (ADCs). BioP

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หมวดหมู่ : HPLC, UHPLC Columns - YMC
รหัสสินค้า : 705

รายละเอียดสินค้า BioPro HIC, Hydrophobic Interaction Column, for protein - YMC

Hydrophobic Interaction Chromatography Column

https://www.ymc.co.jp/en/columns/hic/

Specification

	Matrix	Bonded phase	Particle size (µm)	Usable pH range	Usable temperature range	Characteristics
BioPro HIC HT	Hydrophilic non-porous polymer	Butyl group	2.3	2-12	10-60°C	First-choice HIC column for high resolution/high throughput analyses
BioPro HIC BF	Hydrophilic non-porous polymer	Butyl group	4	2-12	10-60°C	HIC column for separation of low hydrophobic proteins

Comparison of retention behavior

BioPro HIC HT (first choice column) is suitable for successful separation of various biopharmaceuticals.
BioPro HIC BF shows the stronger retention of proteins than BioPro HIC HT due to the stationary phase hydrophobicity, and could be preferred for the separation of low hydrophobic proteins.

Column	100 X 4.6 mmI.D.
Eluent	A) 100 mM NaH2PO4-Na2HPO4 containing 2.0 M (NH4)2SO4 (pH 7.0) B) 100 mM NaH2PO4-Na2HPO4 (pH 7.0) 0%B (0-1 min), 0-100%B (1-11 min), 100%B (11-15 min)
Flow rate	0.5 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	15 µL
Sample	1. Adalimumab (0.5 mg/mL) 2. Trastuzumab (0.5 mg/mL)