BioPro IEX Columns, for proteins, peptides, and nucleic acids - YMC
รายละเอียดสินค้า BioPro IEX Columns, for proteins, peptides, and nucleic acids - YMC
BioPro IEX Columns Features;
-Monoclonal antibody (MAb) analysis on non-porous type cation exchange columns
-Excellent batch to batch reproducibility
-30 mm - length column for high-throughput analysis with low operating pressure
-High binding capacity and high recovery of biomolecules
-High loadability
https://www.ymc.co.jp/en/columns/ymc_biopro/
Specification
| BioPro IEX QA | BioPro IEX SP | BioPro IEX QF | BioPro IEX SF | |
|---|---|---|---|---|
| Matrix | hydrophilic porous polymer beads | hydrophilic non-porous polymer beads | ||
| Particle size (µm) | 5 | 3, 5 | ||
| Charged group | -CH2N+(CH3)3 | -CH2CH2CH2SO3- | -CH2N+(CH3)3 | -CH2CH2CH2SO3- |
| Counter ion | Cl- | Na+ | Cl- | Na+ |
| Ion-exchange capacity [reference value] (meq/mL-resin) | 0.09 | 0.09 | 0.09 | 0.24 |
| Dynamic binding capacity [reference value] (mg/mL-resin) | >110 (BSA) | >70 (human-IgG) | >12 (BSA) | >10 (human-IgG) |
| Usable temperature | 4-60°C | |||
| Usable pH range | 2-12 | |||
| Column material | PEEK | |||
- POINTS
- Newly developed hydrophilic polymer beads with low nonspecific adsorption
- Effective surface structure designed to interact with biomolecules
- High theoretical plate number and symmetrical peak shape by optimal packing technology
- QA (quaternary ammonium) and SP (sulfopropyl) ion exchangers
- Ideal for analysis and laboratory-scale purification on porous type with high binding capacity and high recovery of biomolecules
- Ultra-fast analysis and high-resolution analysis on non-porous type
Monoclonal antibody (MAb) analysis on non-porous type cation exchange columns
The separation of MAb is compared on BioPro IEX SF and competitor's column under the same gradient conditions at pH 5.6.
BioPro IEX SF column provides higher resolution of MAb in a shorter analysis time than the competitor's column.
| Eluent | A) 20 mM MES-NaOH (pH 5.6) B) 20 mM MES-NaOH (pH 5.6) containing 0.2 M NaCl |
|---|---|
| Initial gradient conc. | 35%B (70 mM NaCl） |
| Gradient slope | 0.25%B/min (0.5 mM NaCl) |
| Flow rate | 180 cm/hr (0.5 mL/min for 100 X 4.6 mmI.D., 0.378 mL/min for 250 X 4.0 mmI.D.) |
| Temperature | 30°C |
| Detection | UV at 280 nm |
| Injection | 10 µL |
| Sample | Humanized monoclonal IgG1 (1 mg/mL) |
Excellent batch-to-batch reproducibility
BioPro IEX SF column exhibits excellent batch-to-batch reproducibility for MAb analysis, including the resolution of peaks for small charge variants. All the gel batches are inspected by various quality control tests, including HPLC analysis of MAb, and must pass rigorous criteria before release. BioPro IEX Columns are the best choice for the quality control of MAb and other biopharmaceuticals.
| Column | BioPro IEX SF 5 µm, 100 X 4.6 mmI.D. |
|---|---|
| Eluent | A) 20 mM NaH2PO4-Na2HPO4 (pH 6.5) B) 20 mM NaH2PO4-Na2HPO4 (pH 6.5) containing 0.2 M NaCl 0-50%B (0.5-30 min) |
| Flow rate | 0.5 mL/min (180 cm/hr) |
| Temperature | 25°C |
| Detection | UV at 215 nm |
| Injection | 10 µL |
| Sample | Humanized monoclonal IgG1 |
30 mm-length column for high-throughput analysis with low operating pressure
The separation performance of non-porous and porous columns for proteins is compared. BioPro IEX Colomn non-porous types are effective for high-throughput analysis at a high flow rate because of its high mechanical strength and high column efficiency.
- Ribonuclease A
- Cytochrome c
- Lysozyme
| Eluent | A) 20 mM KH2PO4-K2HPO4 (pH 6.8) B) 20 mM KH2PO4-K2HPO4 (pH 6.8) containing 0.5 M NaCl 0-100%B (0-4 min) for BioPro IEX SF 0-100%B (0-60 min) for BioPro IEX SP |
|---|---|
| Temperature | 25°C |
| Detection | UV at 220 nm |
| Injection | 20 µL |
High binding capacity and high recovery of biomolecules
BioPro IEX QA gives the superior DBC and recovery compared with conventional porous polymer anion exchange columns. The surface structure of porous type BioPro IEX, which is designed for maximum interaction with proteins, provides high binding capacity, and the hydrophilic property of polymer beads significantly reduces nonspecific adsorption of proteins
Comparisons of dynamic binding capacity (DBC) and recovery for BSA
| Dynamic binding capacity (mg/mL-resin, 10% breakthrough) | Eluted amount (mg/mL-resin) | Recovery* (%) | |
|---|---|---|---|
| BioPro IEX QA | 126 | 120 | 95 |
| Brand T (porous Q type) | 73 | 58 | 79 |
| Brand G (porous Q type) | 100 | 35 | 35 |
*Recovery: (Eluted amount/DBC) X 100
Breakthrough curves
| Column | BioPro IEX QA, 50 X 4.6 mmI.D. Brand T (porous Q Type) 50 X 4.6 mmI.D. Brand G (porous Q Type) 50 X 5.0 mmI.D. |
|---|---|
| Linear velocity | 180 cm/hr |
| Equilibration buffer | 20 mM Tris-HCl (pH 8.6) |
| Elution buffer | 20 mM Tris-HCl (pH 8.6) containing 1.0 M NaCl |
| Detection | UV at 280 nm |
| Sample | 1 mg/mL Bovine serum albumin (BSA) in equilibration buffer |
High loadability
BioPro IEX QA shows excellent resolution and peak shapes even when the loading amount increases. The porous type BioPro IEX columns are suitable for laboratory-scale purification of proteins.
Comparison of the effect of sample load on BioPro IEX QA and commercial Q type column
- Ovalbumin
- Trypsin inhibitor
| Eluent | A) 20 mM Tris-HCl (pH 8.1) B) 20 mM Tris-HCl (pH 8.1) containing 0.5 M NaCl 10-80%B (0-30 min) |
|---|---|
| Flow rate | 0.5 mL/min (180 cm/hr for 4.6 mmI.D., 150 cm/hr for 5.0 mmI.D.) |
| Temperature | 25°C |
| Detection | UV at 280 nm |
| Injection | 100 µL |
